| Player | Team | Action | Details | |
|---|---|---|---|---|
| Ryan Zimmerman | Chinballs | ADD | to MLB | 5:18 pm, 6/1/21 |
| Emilio Pagán | Ye Olde Tom Toolshed | ADD | to MLB | 5:00 pm, 6/1/21 |
| Jorge Lopez | Ye Olde Tom Toolshed | ADD | to MLB | 4:58 pm, 6/1/21 |
| Carson Ragsdale | Geckos | ADD | to AA | 1:50 pm, 6/1/21 |
| Cole Henry | Geckos | ADD | to AA | 1:28 pm, 6/1/21 |
| Mitchell Parker | Jon Voight's Car | ADD | to AA | 1:14 pm, 6/1/21 |
| Ethan Elliott | The Bluth Company | ADD | to AAA | 1:13 pm, 6/1/21 |
| Steven Duggar | Chinballs | ADD | to MLB | 10:48 am, 6/1/21 |
| Ivan Herrera | Purple Cobras | ADD | to A | 9:36 am, 6/1/21 |
| Patrick Wisdom | Purple Cobras | ADD | to AAA | 8:39 am, 6/1/21 |
| Ervin Santana | Scranton Yankees | ADD | to MLB | 9:22 pm, 5/31/21 |
| John Gant | Scranton Yankees | ADD | to MLB | 9:21 pm, 5/31/21 |
| Michael Fulmer | Scranton Yankees | ADD | to MLB | 9:20 pm, 5/31/21 |
| Chase De Jong | Scranton Yankees | ADD | to MLB | 9:20 pm, 5/31/21 |
| Jed Lowrie | Scranton Yankees | ADD | to MLB | 9:19 pm, 5/31/21 |
| Cal Raleigh | The Bluth Company | ADD | to AAA | 9:11 pm, 5/31/21 |
| Eric Lauer | Supreme Clientele | ADD | to MLB | 12:42 pm, 5/31/21 |
| Ronnie Dawson | The Bluth Company | ADD | to AAA | 12:37 pm, 5/31/21 |
| M.J. Melendez | The Bluth Company | ADD | to AA | 12:13 pm, 5/31/21 |
| Freddy Galvis | Cowboys From Hell | ADD | to MLB | 9:42 am, 5/31/21 |
| Tucker Barnhart | Cowboys From Hell | ADD | to MLB | 9:42 am, 5/31/21 |
| Caleb Smith | Purple Cobras | ADD | to MLB | 8:30 am, 5/31/21 |
| Lyon Richardson | The Bluth Company | ADD | to A | 12:01 am, 5/30/21 |
| Hudson Haskin | Jon Voight's Car | ADD | to AAA | 7:18 pm, 5/29/21 |
| Alec Burleson | The Bluth Company | ADD | to AAA | 7:17 pm, 5/29/21 |