| Player | Team | Action | Details | |
|---|---|---|---|---|
| Corey Knebel | Scranton Yankees | ADD | to MLB | 2:14 pm, 4/2/21 |
| Elvis Andrus | Scranton Yankees | ADD | to MLB | 2:13 pm, 4/2/21 |
| Gregory Santos | The Bluth Company | ADD | to A | 11:23 pm, 4/1/21 |
| Julian Merryweather | The Bluth Company | ADD | to AAA | 7:30 pm, 4/1/21 |
| Ronald Guzman | Bullet Club | ADD | to AAA | 3:05 pm, 4/1/21 |
| Bobby Bradley | Bullet Club | ADD | to AAA | 2:55 pm, 4/1/21 |
| Bobby Bradley | Bullet Club | ADD | to AAA | 2:54 pm, 4/1/21 |
| Rece Hinds | Bullet Club | ADD | to A | 2:48 pm, 4/1/21 |
| Daniel Cabrera | Jon Voight's Car | ADD | to AA | 1:04 pm, 4/1/21 |
| Levi Kelly | Jon Voight's Car | ADD | to AA | 1:03 pm, 4/1/21 |
| Yoshitomo Tsutsugo | Supreme Clientele | ADD | to MLB | 12:41 pm, 4/1/21 |
| Kyren Paris | Jon Voight's Car | ADD | to A | 12:39 pm, 4/1/21 |
| Oswald Peraza | Cowboys From Hell | ADD | to AA | 12:38 pm, 4/1/21 |
| Alexander Vizcaino | Purple Cobras | ADD | to AAA | 12:38 pm, 4/1/21 |
| Matt Moore | The Red Barons | ADD | to MLB | 12:33 pm, 4/1/21 |
| Tyler Beede | The Red Barons | ADD | to MLB | 12:32 pm, 4/1/21 |
| Jon Berti | Bridge Four | ADD | to MLB | 10:55 am, 4/1/21 |
| Jurickson Profar | Bridge Four | ADD | to MLB | 10:55 am, 4/1/21 |
| Yadier Molina | Ye Olde Tom Toolshed | ADD | to MLB | 10:19 am, 4/1/21 |
| Garrett Cooper | Chinballs | ADD | to MLB | 10:02 am, 4/1/21 |
| Josh Fuentes | Geckos | ADD | to MLB | 2:32 am, 4/1/21 |
| Michael Baumann | The Bluth Company | ADD | to AAA | 1:26 am, 4/1/21 |
| Jhon Torres | The Bluth Company | ADD | to AA | 1:08 am, 4/1/21 |
| William Contreras | The Bluth Company | ADD | to AA | 12:38 am, 4/1/21 |
| Zack Collins | Cowboys From Hell | ADD | to MLB | 7:59 pm, 3/31/21 |